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Make a Wet Mount

Page history last edited by Jay 14 years, 4 months ago

How To Make A Wet Mount

From < http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm#wetmount>

 

1. Gather a thin slice/piece of whatever your specimen is. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw:

 

 

2. Place ONE drop of water directly over the specimen. If you put too much water over the specimen, then the coverslip will float on top of the water, making it harder to draw the specimens as they float past the field of view!

3. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go.

 

 

To see a flash animation of a wet mount go to:

< http://www.wonderhowto.com/how-to/video/how-to-make-a-wet-mount-for-microscope-sample-viewing-259931/view/>

 

How To Stain a Slide

From < http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm#wetmount>

 

 

1. Place one drop of hypertonic soluetion (or stain) on one edge of the coverslip, and the flat edge of a piece of paper towel on the other edge of the coverslip. The paper towel will draw the water out from under the coverslip, and the cohesion of the water (due to those perennial favorites - Hydrogen Bonds) will draw the stain under the coverslip.

 

 

2. As soon as the stain has covered the area containing the specimen you are finished. The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it does.

3. Be sure to wipe off the excess stain with a paper towel, so you don’t end up staining the objective lenses.

4. You are now ready to place the slide on the microscope stage. Be sure to follow all the instructions on the previous pages as to how to use the microscope.

 

 

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