PSOW-4-Investigating SA-V with agar and diffusion

Practical 4 (SA/V)

 

Purpose: Determine affect of SA/V ratio on diffusion out of “cells”

Criteria:  DCP (formative )

Length:   1 hour (max)

Date (time): 

Cohort: 2013 exams

 

 

Objective:

Use conductance as ions leach from agar cubes to determine affect of SA/V on movement in/out of "cells"

 

 

Preparation:

• add 12 g powdered agar per Liter of distilled water
• heat while stirring continuously
• add 100 g NaCl per Liter and mix
• heat until agar is clear - not cloudy or until boiling
• remove from heat and stir for 5 minutes
• pour into dishes and pans

 

 

Method:

[developed by students]