Basic instructions for using Vernier Colorimeter.
Equipment:
- Vernier colorimeter
- (cuvettes with lids-should be in with colorimeter)
- Go-link
Setting Up
(from the Vernier instruction booklet accompanying the instrument):
- Turn on your computer and open Logger Pro 3
- Plug the Colorimeter into Go-Link! and plug Go-Link! into a USB port on your computer. The software should automatically detect the probe and switch to a screen showing "Transmittance" in the lower left corner. Columns will show: Time, Trans, Abs.
- The Colorimeter will take about 5 minutes to warm up. Keep this in mind...
- Rinse the cuvettes inside and outside to make sure they are clean. They are made of polystyrene and can scratch - please do not rub them hard with paper towels, use lens paper.
- Place your sample solutions into your cuvettes. In most cases you need a "standard" for reference so make one and identify it in your data.
- Select the wavelength: 430 nm = violet, 470 nm = blue, 565 nm = green, 635 nm = red
- Select the wavelength closest to the recommended one.
- If a wavelength is not recommended, consider that the color of the solution indicates the light being transmitted by the solution; other colors are being absorbed (try it!).
- Therefore you need to select a different color than the one in the cuvette. Try each wavelength and choose the one with the highest absorbance.
- Place the standard in the Colorimeter. Give it a few seconds to "settle" then press "CAL" on the colorimeter (not the computer). This will zero the instrument to your standard.
- Take several readings using the computer. You should have 15 readings to establish a mean; minimum 7 readings.
- Click on "data collection" in heading of Logger Pro graph
- Change collection time to 30 seconds
- Change collection interval to one collection every 2 seconds (15 collections in total)
- On graph, axis labels can be changed by clicking on axis labels. Popup menu give you options for axis label names. This can be done before or after data collection.
- Click "Start" in upper right hand corner to start data collection. It will stop automatically after the assinged collection period.
- Store each data collection as a unique file name (to keep track of them for later.)
- Save them to easy to find location (like your desktop!)
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